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- Man-Bat (Joker's disguise)
- Real Man-Bat (first animated appearance)(no lines)(cameo)
- Dr. Kirk Langstrom (first animated appearance)(redeemed)
- Alfred Pennyworth (single appearance)
- Guard (single appearance)
- Ventriloquist (cameo)(no lines)
- Poison Ivy (cameo)(no lines)
|Dr. Kirk Langstrom||He is the real Man-Bat.|
|Joker as the Man-Bat||To rob Bruce Wayne's bank account.|
|Frank Welker|| Scooby-Doo|
|Matthew Lillard||Shaggy Rogers|
|Grey Griffin||Daphne Blake|
|Kate Micucci||Velma Dinkley|
|Steven Weber||Alfred Pennyworth|
- This was directed as the first episode of the series, which set the tone for what was to follow.
- Shaggy announces the celebrity guest in the title card. He refers to Batman as "the Batman."
- The episode title is an allusion to the Scooby-Doo, Where Are You! episode What a Night for a Knight.
- This episode has been advertised as the season one finale, even though twenty-six episodes were produced in total.
- Kevin Conroy and Mark Hamill reprise their roles from such series such as Batman: The Animated Series and Justice League Action.
- Steven Weber previously voiced Alfred in the DTVs Batman: Return of the Caped Crusaders and Batman Vs Two Face, based on the Batman 1966 TV series, starring Adam West as Batman.
- On September 9, 2019, Italian news site Angenzia Ansa revealed the title of the episode, the Joker's inclusion, and him being the culprit of dressing up as Man-Bat.
- Fred being pulled into a wall, and Scooby-Doo bouncing through the air and beating on the Man-Bat before knocking him down are references to the Scooby-Doo, Where Are You episode Never Ape an Ape Man, when Fred was fell into a trick library wall, and Scooby attacking the Ape Man, respectively.
- This is the first time Bruce Wayne has appeared in a Scooby-Doo cartoon, thus revealing Batman's identity in Scooby-Doo canon.
- Disguises: Scooby-Doo as a teenage boy.
- In 1970s DC Comics, Alfred Pennyworth had a niece called Daphne Pennyworth.
- Miller & Frank Department Store is named after comic book writer Frank Miller, known for the miniseries The Dark Knight Returns and to the Year One arc in the Batman ongoing.
- The music playing during the title card, and at a few other points during the episode, recalls the Neal Hefti theme to the 1960s Batman TV series.
- While listening to Batman's hold music, Shaggy and Scooby dance to it in the style of "The Batusi" from the 1960s Batman TV series.
Animation mistakes and/or technical glitches
- When she was kidnapped by Man-Bat, Velma had lost her glasses, which are subsequently picked up by Shaggy. But when later found by Batman, she is wearing them again.
Inconsistencies/continuity errors and/or goofs/oddities
- When the fake Man-Bat attacks Alfred, there is no sight of the Joker's wires. But Velma does mention in her mystery explanation that he also used some "controlled gliding"
- How did the Joker know Shaggy's name if they just met?
Shaggy: One of these days, Scoob, I'm gonna to have to teach Freddie the meaning of sarcasm.
Daphne: Boy. This place sure gives new meaning to haunt couture. (Blank looks) It's a fashion joke.
Batman: (Answering phone in the Batmobile) Who is this?
Batman: You're in grave danger. Get out of there now! (Velma drops her phone) Miss Dinkley, Velma, Shaggy-haired hippy guy, talking dog... (ManBat crushes phone)